|
KMID : 0950619970060010039
|
|
Journanl of Allied Health Sciences
1997 Volume.6 No. 1 p.39 ~ p.52
|
|
|
Studies on the Detection of mecA Gene and Distribution of Enterotoxin Genes in Pathogenic Staphylococci Isolated from Korea Using Molecular Biologic Technique
|
|
Whang Sun-Chul
Ryu Jung-Rok
Lee Dong-Hoo
Jung Soo-Kyung
|
|
|
Abstract
|
|
|
|
In this study we attempted multiplex PCR for rapid detection of genes for nuc, mecA. enterotoxin A to E and TSST-l(tst) in bacterial lysates extracted from human strains of S aureus. In 210 isolates of methicillin-resistant Staphylococcus aureus (MRSA), all strains showed both nuc gene and mecA gene positive except two strains that showed only nuc gene positive. One hundred sixty nine(80.5%) of 210 MRSA isolates tested produced one or more toxins. Other 41(19.5%) isolates did not produce any of the toxins. Enterotoxin A gene(sea) was amplified in 50.5%(n=105) of the isolates, enterotoxin B(seb) in 14.3%(n=30), enterotoxin C(sec) in 23.3%(n=49), enterotoxin D(sed) in 10.0%(n=21) and TSST-l(tst) in 12.4%(n=26). Fifty eight strains(27.6%) amplified more than two enteroxin genes. The most common combination of toxin gene was sea and seb, which was found in 26(12.4%) strains, and the next frequent combination was sec and tst in 14(6.7%) strains from 210 isolates among which 109 MRSA strains tested were isolated in Seoul area and 101 in Pusan area. The frequency rate of enterotoxin A in Pusan was twice higher than that of Seoul with 57.1% to 23.6%. But the frequency rates of enterotoxins C and D genes in Seoul area are analogous with relatively narrow margin while those rates in Pusan area were almost negligible with less than 2% each. Apparent difference of enterotoxin type distribution was also found between the countries. Beside enterotoxin A which was most predominantely found in both European countries and Korea, enterotoxin C was the subsequently prevalent type of toxin in Korea instead of enterotoxin b which was found as the second most prevalent enterotoxin in some European countries.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|